UMP-CMP kinase/CMPK1 His Tag Protein, Human

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货号： UA070016

大包装询价

产品介绍引用文献(0) 评论(0)

产品规格

物种

Human
分子别名

CK, CMK, CMPK, UMK, UMP-CMPK, UMPK
Accession

P30085
表达序列

Met1-Gly196, with N-terminal 8*His HHHHHHHHGGGSMKPLVVFVLGGPGAGKGTQCARIVEKYGYTHLSAGELLRDERKNPDSQYGELIEKYIKEGKIVPVEITISLLKREMDQTMAANAQKNKFLIDGFPRNQDNLQGWNKTMDGKADVSFVLFFDCNNEICIERCLERGKSSGRSDDNRESLEKRIQTYLQSTKPIIDLYEEMGKVKKIDASKSVDEVFDEVVQIFDKEG
表达宿主

Baculovirus-InsectCells
分子量

26-29kDa
纯度

>95% by SDS-PAGE
内毒素含量

<0.1EU/μg
标记

Unconjugated
标签

His Tag
性状

Liquid
缓冲体系

20mM Tris, 500mM NaCl,10%Glycerol, pH7.4
溶解方法

Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件

12 months from date of receipt, -20 to -70 °C as supplied; 6 months, -20 to -70 °C under sterile conditions after reconstitution; 1 week, 2 to 8 °C under sterile conditions after reconstitution; Please avoid repeated freeze-thaw cycles.
文献引用

1、Liou J. et al. (2004) Phosphorylation of Cytidine, Deoxycytidine, and Their Analog Monophosphates by Human UMP/CMP Kinase is Differentially Regulated by ATP and Magnesium. Molecular Pharmacology. 67(3): 806-14.
2、Gerhard D S. et al. (2004) The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC). Genome Research. 14(10B): 2121-7.
3、Segura-Pena D, et al. (2004) Substrate-induced Conformational Changes in Human UMP/CMP Kinase. Journal of Biological Chemistry. 279(32): 33882-9.

背景介绍

Pyrimidine nucleoside monophosphate kinase [UMP/CMP kinase (UMP/CMPK)] (CMPK1) is also named as CMK, CMPK, UCK, UMK, UMPK and belongs to the adenylate kinase family. The gene of encodes one of the enzymes required for cellular nucleic acid biosynthesis. CMPK1 plays an important role in de novo pyrimidine nucleotide biosynthesis. This enzyme catalyzes the transfer of a phosphate group from ATP to CMP, UMP, or dCMP, to form the corresponding diphosphate nucleotide. Alternate splicing results in both coding and non-coding transcript variants. CMPK1 has been reported as a prognostic marker for non-small cell lung cancer, pancreatic cancer and breast cancer. It has 3 isoforms with the molecular mass of 22, 16 and 26 kDa. The longest deduced protein contains 228 amino acids and has a calculated molecular mass of 26 kD.

生物活性
- When UMP-CMP kinase/CMPK1 His Tag, Human is immobilized at 0.2µg/mL, it binds to rabbit anti UMP-CMP kinase pAb with an EC50 of 0.010 to 0.013µg/ml.
电泳(SDS-PAGE)
- 2μg (R: reducing conditions).