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物种
Human分子别名
Fibronectin, FN, CIG, FNZ, MSFAccession
P02751-15表达序列
Pro1361-Ser1637&Ala1812-Thr2107表达宿主
E.coli分子量
58kDa (Reducing)
纯度
＞95% by SDS-PAGE& HPLC内毒素含量
<0.1EU/μg标记
Unconjugated标签
No Tag性状
Lyophilized Powder缓冲体系
20mM Tris, 200mM NaCl, 5% Trehalose, 0.02% Tween-20, pH8.5溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.文献引用
Zhou Y. et al. (2020) The effect and clinical significance of FN1 expression on biological functions of gastric cancer cells. Cell Mol Biol (Noisy-le-grand). 66(5): 191-198.
FN (Fibronectin) is a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. It is proteolytically processed to generate the mature protein. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. In recent years, FN has been shown to play an important role in the regulation of various malignant tumors such as lung cancer, colorectal cancer, and ovarian cancer.
生物活性
Measured by the ability of the immobilized protein to support the adhesion of NIH-3T3 mouse embryonic fibroblast cells. The EC50 for this effect is less than 120ng/ml.
电泳(SDS-PAGE)
1μg (R: reducing condition, N: non-reducing condition).
反相高效液相色谱(RP-HPLC)
>95% as determined by RP-HPLC.
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