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物种
Human分子别名
OSM, Oncostatin M, MGC20461, OncoMAccession
P13725表达序列
Ala26-Arg234表达宿主
E.coli分子量
24kDa纯度
>95% by SDS-PAGE内毒素含量
<1EU/μg标记
Unconjugated标签
No Tag性状
Lyophilized Powder缓冲体系
20mM Tris, 150mM NaCl, pH8.0, 1mM TCEP溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.储存条件
12 months from date of receipt, -20 to -70 °C as supplied;
6 months, -20 to -70 °C under sterile conditions after reconstitution;
1 week, 2 to 8 °C under sterile conditions after reconstitution;
Please avoid repeated freeze-thaw cycles.
文献引用
1、Tanaka M. et al. (2003) Oncostatin M, a multifunctional cytokine. Rev Physiol Biochem Pharmacol. Reviews of Physiology, Biochemistry and Pharmacology. 149: 39-52.
2、Auguste P, et al. (1997) Signaling of type II oncostatin M receptor. J Biol Chem. 272(25): 15760-15764.
Oncostatin M (OSM) was initially identified as a polypeptide cytokine which inhibited the in vitro growth of cells from melanoma and other solid tumors. OSM shows significant similarities in primary amino acid sequence and predicted secondary structure to leukemia inhibitory factor (LIF), ciliary neurotrophic factor (CNTF), granulocyte colony-stimulating factor (G-CSF), interleukin 6 (IL-6), and interleukin 11 (IL-11). The antiproliferative activity of oncostatin M for some cell lines is synergised by TGF-beta and IFN-gamma. It promotes the growth of human fibroblasts, vascular smooth muscle cells, and some normal cell lines. Other OSM-mediated activities reported to date include: stimulation of plasminogen activator activity in cultured bovine aortic endothelial cells; regulation of IL-6 expression in human endothelial cells; and stimulation of LDL uptake and up-regulation of cell surface LDL receptors in HepG2 cells.
生物活性
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Measured by a cell proliferation assay using TF-1 cells, The EC50 for this effect is less than 1ng/ml.
电泳(SDS-PAGE)
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1μg (R: reducing condition, N: non-reducing condition).
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