GM-CSF Protein, Human

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货号： UA040002

大包装询价

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产品规格

物种

Human
分子别名

GM-CSF
Accession

P04141
表达序列

Ala18-Glu144
表达宿主

CHO
分子量

20-25 kDa(Reducing)
纯度

＞95%, by SDS-PAGE under reducing conditions
内毒素含量

<0.1EU/μg
标记

Unconjugated
标签

No Tag
性状

Lyophilized Powder
缓冲体系

PBS, pH7.4
溶解方法

Reconstitute at less than 1 mg/mL according to the size in ultrapure water after rapid centrifugation .
储存条件

· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.

背景介绍

Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as colony-stimulating factor 2 (CSF2), is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, natural killer cells, endothelial cells and fibroblasts that functions as a cytokine. GM-CSF was first described as a growth factor that induces the differentiation and proliferation of myeloid progenitors in the bone marrow, which also has an important cytokine effect in chronic inflammatory diseases by stimulating the activation and migration of myeloid cells to inflammation sites, promoting survival of target cells and stimulating the renewal of effector granulocytes and macrophages. GM-CSF receptor is composed of one α chain and one β chain with low and high-affinity binding to GM-CSF, respectively, and the β chain is shared with IL-3 and IL-5 receptor. GM-CSF signals via signal transducer and activator of transcription, STAT5. In macrophages, it has also been shown to signal via STAT3. The cytokine activates macrophages to inhibit fungal survival.

免疫印迹
- Primary antibody: CD209 gamma Recombinant Rabbit mAb (S0B1390) at 1/1000 dilution
  Lane 1: untreated THP-1 whole cell lysate 20 µg
  Lane 2: THP-1 treated with 100 ng/ml GM-CSF (UA040002) and 50 ng/ml hIL-4 (UA040026) for 7 days whole cell lysate 20 µg
  Lane 3: THP-1 treated with 100 ng/ml GM-CSF (Competotor T) and 50 ng/ml hIL-4 (UA040026) for 7 days whole cell lysate 20 µg
  Lane 4: THP-1 treated with 100 ng/ml GM-CSF (UA040002) and 50 ng/ml hIL-4 (Competitor P) for 7 days whole cell lysate 20 µg
  Lane 5: THP-1 treated with 100 ng/ml GM-CSF (Competotor T) and 50 ng/ml hIL-4 (Competitor P) for 7 days whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 46 kDa
  Observed MW: 45~55 kDa
生物活性
- EC50 < 0.1ng/ml, measured in a cell proliferation assay using TF-1 cells
- Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The EC50 for this effect is less than 0.1ng/ml.
电泳(SDS-PAGE)
- GM-CSF, Human，3μg on SDS-PAGE under Non-reducing and reducing condition. The purity is greater than 95%.
SPR
- Anti-His antibody Immobilized on CM5 Chip captured GM-CSFR α His Tag, Human (Cat. No. UA010189), can bind GM-CSF, Human (UA040002) with an affinity constant of 11.9 nM as determined in SPR assay.