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Mouse IgG (Total) Broad range ELISA Kit

货号: S0C3038

  • 价格: ¥3,200
  • 规格:
  • 数量:
斯达特大包装询价 大包装询价

产品介绍 引用文献(0) 评论(0)

产品规格
  • 抗原名称

    IgG (Total)
  • 免疫原

    Recombinant Protein
  • 抗体类型

    Recombinant mAb
  • 反应种属 ?

    Ms
  • 纯化方式

    Protein A
  • 储存条件

    12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.

  • 稀释度

    [{"application": "ELISA", "dilution": "", "species": ""}, {"application": "Sandwich ELISA", "dilution": "", "species": ""}, {"application": "CLIA", "dilution": "", "species": ""}, {"application": "Lateral Flow", "dilution": "", "species": ""}, {"application": "Dot Blot", "dilution": "", "species": ""}, {"application": "WB", "dilution": "", "species": ""}, {"application": "IP", "dilution": "", "species": ""}, {"application": "IHC", "dilution": "", "species": ""}, {"application": "ICC", "dilution": "", "species": ""}, {"application": "IF", "dilution": "", "species": ""}, {"application": "mIHC", "dilution": "", "species": ""}]
试剂盒参数
    • Precision

      Intra-assay: 5.2%;
      Inter-assay: 8.8%
    • Sample type

      Cell culture supernatant, Serum, Plasma
    • Assay type

      Sandwich (quantitative)
    • Sensitivity

      0.046ng/ml
    • Range

      2.05 ng/mL –500 ng/mL
    • Recovery

      Cell culture supernatant: 83%
      Serum: 92%
      Plasma:112%
    • Assay time

      45 minutes
    • Species reactivity

      Mouse
背景介绍
  • Mouse IgG is the most abundant antibody isotype in humoral immunity, composed of two γ-heavy chains (γ-chains) and two light chains (κ or λ) linked by disulfide bonds to form a canonical Y-shaped monomeric structure. The γ-heavy chain contains three constant domains (CH1–CH3), with the Fc region (CH2–CH3) mediating binding to Fcγ receptors (FcγRI/II/III) and complement protein C1q. Unlike IgE, IgG lacks the Cε1 domain but features a hinge region that confers molecular flexibility, enhancing antigen-binding diversity. Glycosylation modifications (e.g., N-linked oligosaccharides in the Fc region) regulate IgG effector functions, such as antibody-dependent cellular cytotoxicity (ADCC). Applications of the Mouse IgG ELISA Kit: This kit is designed for quantitative detection of total IgG in mouse serum, plasma, or cell culture supernatants. Advantages of this OneStep ELISA Kit Design: Simplified Workflow: Combines sample incubation and detection in a single step, eliminating intermediate washing and reducing hands-on time. High Efficiency: Achieves results within 45 minutes, ideal for high-throughput studies or urgent diagnostics. Enhanced Precision: Minimizes procedural variability and cross-contamination risks through optimized reagent formulation. Broad Compatibility: Validated for diverse biological matrices, ensuring flexibility in experimental design. This kit’s streamlined protocol and robust performance make it an indispensable tool for researchers and clinicians seeking reliable IgG quantification in time-sensitive or resource-limited settings.

  • ELISA

    • Standard curve
      Example of Mouse IgG standard curve in Assay Diluent #1.

    • Linearity
      The concentrations of Mouse IgA were measured and interpolated from the target standard curves and corrected for sample dilution.
      #1 sample is undiluted samples are as follows: Mouse Serum was 4x10^4fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 3.14 mg/mL in Mouse Serum.

    • Linearity
      #2 sample is undiluted samples are as follows:  Mouse Plasma was 1x10^4fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 1.30 mg/mL in Mouse Plasma.

    • Protocol Diagram

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