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Rabbit抗原名称
HSC70分子别名
Heat shock cognate 71 kDa protein; Heat shock 70 kDa protein 8; Heat shock protein family A member 8; Lipopolysaccharide-associated protein 1 (LAP-1, LPS-associated protein 1); HSPA8; HSP73; HSPA10免疫原
Synthetic Peptide细胞定位
Cytoplasm, Nucleus, Cell membraneAccession
P11142克隆号
S-996-65抗体类型
Recombinant mAb抗体同种型
IgG应用
ICFCM, ICC, WB反应种属 ?
Hu, Ms, Rt预测反应种属
(反应种属缩写表)Bv, Av, Hr, Or, Hm, Rb纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 |
|---|---|
| WB | 1:1000 |
| ICC | 1:500 |
| ICFCM | 1:500 |
Heat shock 70 kDa protein 8 also known as heat shock cognate 71 kDa protein or Hsc70 or Hsp73 is a heat shock protein that in humans is encoded by the HSPA8 gene. As a member of the heat shock protein 70 family and a chaperone protein, it facilitates the proper folding of newly translated and misfolded proteins, as well as stabilize or degrade mutant proteins. Its functions contribute to biological processes including signal transduction, apoptosis, autophagy, protein homeostasis, and cell growth and differentiation. It has been associated with an extensive number of cancers, neurodegenerative diseases, cell senescence, and aging.
免疫印迹
WB result of HSC70 Recombinant Rabbit mAb
Primary antibody: HSC70 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: K562 whole cell lysate 20 µg
Lane 3: A431 whole cell lysate 20 µg
Lane 4: MCF-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 71 kDa
Observed MW: 70 kDaWB result of HSC70 Recombinant Rabbit mAb
Primary antibody: HSC70 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 71 kDa
Observed MW: 70 kDaWB result of HSC70 Recombinant Rabbit mAb
Primary antibody: HSC70 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 71 kDa
Observed MW: 70 kDa
流式分析
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized MCF7 (Human breast adenocarcinoma epithelial cell) labelling HSC70 antibody at 1/500 dilution (0.1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫细胞化学
ICC shows positive staining in MCF-7 cells. Anti-HSC70 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
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